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Image Search Results
Journal: Integrative biology : quantitative biosciences from nano to macro
Article Title: Stiffness of pancreatic cancer cells is associated with increased invasive potential
doi: 10.1039/c6ib00135a
Figure Lengend Snippet: (A) Time series of images showing scratch wound invasion of pancreatic ductal cells through Matrigel. Wound confluence is the percentage of wound area covered by cells. Color legend: green is the wound area, blue shows wound confluence in the wound area, and grey represents the confluent cells outside of the wound area. Scale, 300 μm. (B) Schematic illustration showing the modified scratch wound assay. Cells are plated on a layer of thin Matrigel and invade into the thick 3D matrix of overlaid Matrigel that fills the scratch wound. The line plot shows quantification of wound confluence over time. The dotted line indicates the 72 h time point, which we use to compare wound confluence values for statistical significance. The bar plot represents wound confluence at the 72 h time point. Pairwise p-values are determined by a Student’s t-test. *p < 0.05. (C) Schematic illustration showing the transwell migration assay. Cells migrate through the 8 μm pores of a polycarbonate membrane in response to a chemoattractant on the opposite side of the membrane. Images of transwell migration assays showing DRAQ5-labeled nuclei of cells that migrate through the 8 μm pores of a polycarbonate membrane after 12 hours. Scale, 50 μm. Pairwise p-values are determined by a Student’s t-test. *p < 0.05. All error bars represent standard errors. The significance of pairwise comparisons between cell lines is shown in panels B and C by the initial(s) of the cell lines that are significantly different where H: HPDE, Hs: Hs766T, M: MIA PaCa-2, and P: PANC-1. For example, in panel B, HPDE is significantly different (*p < 0.05) from Hs766T (Hs) and PANC-1 (P).
Article Snippet: To image the nucleus, cells are stained with
Techniques: Modification, Scratch Wound Assay Assay, Transwell Migration Assay, Migration, Labeling